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Sample Collection and Submission Guide
Samples are recorded and
processed routinely by the date and time in which they are received. All
samples will be initially processed within five days of receipt with results
available twenty-one days after processing. Some laboratory diagnostic
techniques take longer than others, which may affect result punctuality.
Sample Collection
Tree disease diagnosis is
largely dependent on the quality of the sample and on the relevant
information provided by the submitter. Samples must be of sufficient
quality and quantity to allow for proper laboratory testing and pertinent
information, such as sample tree identification, is essential.
1. Collect samples
before the application of pesticides in order to increase the probability of
recovering the causal pathogens.
2. Samples should be
collected from symptomatic trees showing thinning/transparent crowns,
foliage discoloration, and/or excessive cone production.
3. Samples should not be
collected from dead trees; determining the causal agent from such trees is
highly unlikely. Samples should not be collected from dead roots for the
same reason.
4. Submit a generous
amount of sample material to allow for all required laboratory processes.
Collect 12 to 18 inches of first order lateral roots that are 3/4 to 4
inches in diameter. Be sure to include insect damaged and stained
roots.
5. Carefully excavate to
avoid unnecessary damage to the sample.
6. Excess soil should be
removed from root samples. Samples should retain just enough soil to
maintain moisture levels.
7. Wrap samples in dry
paper towel and seal in a zip-top bag and place on ice. Do not add
water or pack a sample in water.
8. Keep samples
refrigerated from collection to submission and do not expose them to high
heat situations, such as baking in the sun or in the back of a vehicle.
9. Keep all samples in
separate bags and label appropriately.
10. Clean and sterilize
all sampling tools between the collection of different samples to prevent
spreading the pathogen from one tree to another.
11. Complete a "Tree Disease Diagnostic Form " for each sample.
Sample Submission
Samples may be delivered in
person or mailed to the, Forest Health Dynamics Laboratory,
602 Duncan Drive, Ste 3301, Auburn University, AL 36849-5418.
1. All samples MUST be submitted with a
completed "Tree Disease Diagnostic Form."
2. When submitting
samples by mail, either mail them early in the week to avoid weekend
layovers or use an overnight service. You may also deliver them in
person.
3. Samples should be
mailed in an appropriate sized box, with padding, or in a padded envelope.
Please write on the outside of the package "Refrigerate Upon Arrival."
4. If sample is a
suspected high-risk pathogen, please contact Forest Health Dynamics
Laboratory personnel for appropriate packaging and mailing instructions.
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