Pitch Canker Screening of Seed Lots and Seedling Samples
Advances in molecular techniques have allowed for the successful identification and quantification
of pathogens affecting seed health. The Nursery Cooperative have recently developed a molecular
technique that can identify Pitch Canker from bulk seed DNA extraction without the need to
initially culture the Pitch Canker from tested seed / seedlings.
The DNA extraction test can be used as a rapid screening tool for quality control testing for
Pitch Canker. We have submitted our protocol to the International Seed Testing Authority for
consideration as an official method. Our intention is that once the molecular method is approved
by the ISTA, that it be used to rapidly screen pine seed lots to assist in the reduction of Pitch
Canker spread.
Although this method has not as yet been approved by the ISTA, we have decided to begin seed lot
and/or seedling screening service to our members. Please contact me before sending your samples
as we want to ensure that we can process your seed/seedlings in an expedient manner.
Listed below are some points that cover most of the information you need to know:
- One sample = 400 seed from a single seed lot OR 1 seedling.
- Cost - $35 per sample.
- We will send you an invoice once analyses have been completed.
- Multiple samples can be shipped together as long as they are clearly identified.
- A "Pitch Canker Screening Submission Form" must accompany each sample.
- Ship seed/seedlings dry – no ice. Ship so they arrive in 2 days and are NOT in transit over the weekend.
Communication between your company and the Nursery Cooperative is key to the success of this project. Call me about any questions, concerns or suggestions at any time. Call me about how to collect each sample if needed. -- Ryan Nadel, 334-844-8069